Nanobio Research

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General Information

Nanobio contains 17 different strands of micro-organisms. They are at the same time aerobic and anaerobic; this means the whole mix can sustain itself: aerobic microbes consume oxygen and discharge CO2, while anaerobe microbes use CO2 and discharge oxygen.

  1. Photosynthetic Bacteria The photosynthetic or phototropic bacteria are a group of independent, self supporting microbes. These bacteria synthesize useful substances from secretions of roots, organic matter and/or harmful gases (eg. hydrogen sulphide), by using sunlight and the heat of soil as sources of energy. Useful substances developed by these microbes include amino acids, nucleic acids, bioactive substances and sugars, all of which promote plant growth and development. The metabolites developed by these microorganisms are absorbed directly into plants and act as substrates for increasing beneficial populations.
  2. Lactic acid bacteria Lactic acid bacteria produce lactic acid from sugars and other carbohydrates, developed by photosynthetic bacteria and yeast. Therefore, some foods and drinks such as yogurt and pickles have been made with lactic acid bacteria for decades. However, lactic acid is a strong sterilizing compound, and suppresses harmful microorganisms and enhances decomposition of organic matter. Moreover, lactic acid bacteria promote the decomposition of material such as lignin and cellulose and ferments these materials, thereby removing undesirable effects of undecomposed organic matter.
  3. Yeast Yeasts synthesize antimicrobial and other useful substances required for plant growth from amino acids and sugars secreted by photosynthetic bacteria, organic matter and plant roots. The bioactive substances such as hormones and enzymes produced by yeasts promote active cell and root division. These secretions are also useful substrates for effective microbes such as lactic acid bacteria and actinomycetes.
  4. Actinomyces (are between bacteria and fungi; create anti-microbial substances that suppress harmful fungi and bacteria; they help nitrogen assimilation on roots of legumes)
  5. Fermentative fungi (aspergillus, penicillium decompose organic material to produce alcohol and anti-microbial substances; they suppress odours and vermin)

Nanobio produces a vast quantity of antioxidants, enzymes and healthy electromagnetic frequencies that can erase harmful memory in water.

The micro-organisms become active at 6ºC; their ideal temperature lies between 25-37ºC. Nanobio has a pH of 3.2-3.9 (a little less than vinegar/coke); it needs to be stored in warm and dark place.

The compounds of Effective Microorganisms:

The lactic acid bacteria (LAB) produce lactic acid as the major metabolic end product of carbohydrate fermentation. LAB are also characterized by an increased tolerance to a lower pH range. This enables LAB to out-compete other bacteria in a natural fermentation, as they can withstand the increased acidity from organic acid production. Through the metabolism of LAB, CO2 (carbon dioxide) is formed. This is used by other species in the consortia as a source of energy to their own metabolic systems, e.g. phototrophic bacteria. The yeast and phototrophic bacteria are known as heterotrophic bacteria, meaning they use organic substrates to get carbon for their growth and development. Heterotrophic bacteria can reproduce in as little as 15 minutes to 1 hour (Biocon Labs, 2008). Heterotrophic bacteria, such as Bacillus are also very resilient in the environment due to their heat-resistance characteristics and spore-forming abilities, which help to increase shelf-life.

Phototrophic bacteria (or PNSB) are capable of using both organic and inorganic materials as hydrogen donors throughout their growth cycle. The ability of PNSB species to use hydrogen sulfide, which is toxic, and convert it into nonpoisonous compounds is also very beneficial in the waste water industry (Kobayashi and Kobayashi, 2002).

The yeast have the ability to assimilate glucose as a substrate and produce pyruvic acid through metabolism of the saccharide decomposed system. Pyruvic acid can be used as a substrate of microaerobic lactic acid bacteria. In this way, if the lactic acid bacteria using the metabolite of yeast multiply, the formed lactic acid becomes the substrate of photosynthetic bacteria and they can be multiplied. Then yeast uses the saccharides formed by this photosynthetic bacteria as a substrate and

can multiply repeatedly. This implicates that the microbes continue to aid each other to keep alive and stay strong in the environment.

Quang (2006) reported that the species in Nanobio, including Lactobacillus, Yeast (such as Bacillus), Rhodopseudomonas and other beneficial species, have shown to reduce total coliform counts in wastewater facilities by 80% after 4 months of treatment. This is a dramatic reduction in total coliforms. The metabolites from LAB have shown anti-microbial ability in some studies. Depending on the environment, nutrition and type of species, they can produce lactic acid, acetic acid, ethanol, diacetyl, CO2;(as carbonic acid), H2O2, reuterine, derivatives of lactic acid (hydroxyl lactic acid), and small peptides (such as bacteriocins) (Ray, 2000; Nes and Holo, 2002).

These antimicrobial agents can either inhibit or kill target microorganisms such as molds, yeasts, coliforms, vegetative bacteria, bacterial spores, and even viruses.

In one case, after replacing oxygen injections with SCD Probiotics Technology, the hydrogen sulfide levels decreased significantly and oxygen injections were discontinued (Boyd, 1999). The results seen included the immediate reduction of odors within 24 hours. This is due to the microbes ability to degrade various organic compounds. Consortium microbes are able to digest organic compounds and release beneficial by-products.

By using SCD Probiotics Technology, Indonesia showed reductions of COD, BOD and SS of 40%-50%, 42%-55% and 44%-71% respectively (Wididana, 2006). SCD Probiotics Technology was applied periodically every 10 days. The microbes were able to ferment the organic matter resulting in the formation of simpler organic compounds, such as amino acids, alcohols, sugars and organic acids that are more beneficial and not harmful to the surrounding environment. This results in a reduction of COD, BOD and SS that typically would result in putrefactive odors.

The phototrophic bacteria in SCD Probiotics Technology also has an extremely important role for wastewater management due to their ability to degrade organic compounds. They can oxidize NH3 and use the natural sunlight as a source of energy and CO2 as a source of carbon (Wididana, 2006).

As mentioned earlier, by having multiple types of bacteria in one culture, SCD Probiotics Technology products are able to degrade various organic compounds, rather than one single strain of microorganism. When levels of BOD, COD, and SS increase, this increase indicates a decrease in oxygen which releases foul odor. When these levels are extremely high, the organic compounds cannot be degraded biologically alone (Wididana, 2006). SCD Probiotics Technology can aid in this problem by digesting the organic compounds and releasing beneficial by-products, such as amino acids as mentioned previously.

Waste Water Treatment

Sewage Treatment, Macay, Queensland, Australia

Due to increased load on the sewerage system in recent years Mackay has experienced a growing problem with sewerage odour. This has resulted in a concerted and very public attempt to find a community-wide solution. The odour problem is centred on effluent arriving at the city’s main Sewerage Treatment Facility and at each of the main Pumping Stations across the region.

It involved setting up a series of low dose, widespread, accumulative inoculation points at which EM formulations are injected. A pattern of inoculation was established such that all effluent in the system if inoculated at least once well before reaching the sewerage treatment plant (VRM, 19991).Initial trials focussed on an area of Mackay’s sewerage system known as the Beaconsfied collection system which collects on average approximately 2.4 megalitres of mixed effluent per day.
Thirteen inoculation points were selected and equipment installed at each included a specially constructed storage and equipment cabinet and metered dosing equipment capable of continuous inoculation at rates as low as 100 ml per hour.
Extended EM formulation were prepared and diluted at four parts to one with aged (de-chlorinated) water. The resulting mixture was delivered in an even amount to each inoculation site such that a total inoculation of approximately 100 ppm inoculum to waste flow was achieved across the system.
Dosing began on 29 March 1998. After one month, dosing rates were reduced to approximately 25 ppm and thereafter, were progressively reduced to a low of approximately 1.5 ppm. In February 1999, dosing rates were increased to approximately 15 ppm in an attempt to address indicators other than hydrogen sulphide. After a period of one month the inoculation rate was reduced again to 2.5 ppm and continues at that level to date.
In July 1998 the trail was extended to include a second section of the system known as the Slade Point collection system. A further ten inoculation sites were chosen and similar equipment installed. Dosing began on 15 July 1998.
Inoculation sites take two forms : (a) Injection above the influent stream at an existing pumping station; (b) Injection to a specially constructed in-line biological filter (VRM, 1999). Injection is by way of specially designed nozzles which allow partial aerosoling of liquid and a simultaneous full cone droplet spray.
Installation of the spray nozzle is done so as to achieve both a mixture of EM with all effluent in the situation and mist of droplets floating in the ullage space provided above the effluent.
Biological filters contain a quantity of EMX ceramic media which is placed so that effluent passes over and through the ceramic. Inoculation was continued on a 24 hour per day basis and cannister refills were completed on a 10 day rotation.

The immediate and most notable result of the process was that all detectable sewerage odour normally generated from the system ceased within 24 hours of commencement of inoculation. This was supported by an immediate drop in water-borne hydrogen sulphide readings to a steady reported level of less than one mg/l.
Early in the trial, community monitors reported the appearance of a different odour. This was identified as being odour generated by the EM itself (a sweet fermentation odour). As dosing rates were decreased this odour diminished and is now not detectable.
Fat build-ups which normally plague most local authority sewerage systems where observed to be significantly reduced. This resulted in much reduced costs for labour in periodic cleaning/removal of fats. It has been noted that fats do not reconstitute downstream of inoculation points and that build-up of fats does not re-occur once cultures of EM are observable in the sewerage system. Any residues are easily hosed from the walls of structures and do not subsequently require mechanical scraping or other removal techniques.
These trials concluded that where blanket pre-treatment has occurred, the level of DO present in effluent at the end of long rising mains was at least equivalent to that present where direct oxygen injection was undertaken.



Guatemala City and Amatitlan Lake

nano3In this case, the waste water collection site is part of the municipal trash dump; there was no electricity and no clean running water supply; so pumping etc was not possible.

Prof. Higa, the developer of the microorganism technology used therefore a gravitational fed array of inoculated basins to clean the waste water.

On his arrival the totality of the waste water from Guatemala City (3 M at that time) went essentially untreated into the Amatitlan lake, an important tourist attraction and drinking water reservoir of Guatemala. Because of the organic charges, the lake had develop numerous blue-green algae blooms (cyanobacter), very low transparency and foul odors. During the dry season all of the sewage of Guatemala City passes through this unit; and 10% during the rainy season.

nano6The installation consists of 3 basins of 5 ha (12 acres) surface each; each of them has been inoculated with 2000-3000L Nanobio1 (activated microorganisms) weekly. A natural overflow leads the water into a last basin with water purification plants, where the water stays for 3-4days before it discharges into a river that flows into the Amatitlan lake. After an inoculation of nine month the foul odor was gone, the water was clear again, no algal blooms have been observed since and the BOD has been reduced by 75%.


Gushigawa Library, Okinawa/Japan

nano5This public library produces an average of 32m³ of sewage per day; the design maximum is for 6.83m³ per hour.

The total amount of sewage is treated to non-potable uses like flushing toilets (Nanobio prevents deposits in the toilets); to clean carpets (Nanobio prevents build-up of black mold – in the humid and hot climate of Okinawa); irrigation (with additional use of reclaimed rainwater); the metal parts of the equipment stay mainly corrosion-free which diminishes maintenance costs.


NANOBIO-SEWAGE-MIX (activated microorganisms) is injected once every two weeks into the collector 1 tank at a rate of 14L; after 8 weeks the rate is changed to 3L once a month.

The aeration rate in the first year has been 3hrs/day; from year 3 onwards 2hrs/day.


  1. electricity costs: the reduction of aeration from 24/7 to 3hrs and subsequently 2hrs/day reduced the electrical bill by 95%
  2. water costs have been reduced by 92% from 12,000.00$/year to 600.00$/year
  3. further reductions have been achieved by the fact that sludge removal has not been necessary; less corrosion on fittings etc
  4. the BOD (biological oxygen demand) has been dimished by 85%, from 20ppm (legal allowance for non potable applications in Japan) to less than 3ppm.
  5. E-coli have been dimished by 92.5% from 12.000 cfu to 900


(a) To control foul odors in the sewage system escaping from the manholes area, reducing complaints from surrounding neighbors
(b) To reduce the levels of Hydrogen Sulfide (H2S) to within the legal guidelines (<35ppm).

The overall length of the sewage system was 21 km, and the total retention time was 2.5 hours (approximate time the products had to impact the waste water quality); charge varies between 210-3,100m³/day.

700L of NANOBIO 2 were applied directly into the process before the aeration station



Reseda Lake Park, Los Angeles, California, USA

nano9Customer Problem
Reseda Lake is a man-made lake, approximately 2.5 acres in size and 9 feet deep, in a recreational area of Reseda Park. The lake is polluted from the droppings of birds and the decomposition of food that has been scattered in the water by visitors who feed the birds (see chart below for baseline pathogen measurements).

The lake water in Reseda Lake should meet or exceed the city standards for lake water quality, consistent with the standards established by the Department of Natural Resource (see chart below for standards the pilot project was tasked to achieve).

A baseline measure of metals and microbes were established in October 2008 prior to the treatment using SCD Probiotics products and technology (see chart below). All samples were analyzed for total, E. Coli, Enterococcus and Total Colifoms bacteria, as well as copper. The microbial culture formulation and application process designed and developed by the SCD Probiotics technology team in partnership with California-based Organic Environmental Technology.
Product applications began in March 2009. A solid microbial culture (Magic Sinkers) were placed at various strategic locations along with activated wood charcoal (Impregnated Carbon) and a liquid microbial culture (Pond Magician) was injected at the aerator unit. Samples at six locations around the lake were taken on June 24, 2009.

The table below represents the significant improvement in water quality on all levels, with sample results testing far below the City Standards.

*Average of six samples taken prior to the start of the SCD Probiotics applications, in north, south, east, west and center of the lake
**South side of Reseda Lake is where the majority of the ducks, geese and other bird life enter the lake.

Cartagena, South America

Wastewater ‐ Controlling Odor and Reducing Bacterial Load3

Customer Problem
The Cartagena tourist area faced foul odors coming from the public manholes of the urban sewerage wastewater, with a health concern especially in the rainy season when the manholes overflowed.
The project concentrated on three geographic areas, served by one pumping station – with a pumping volume of about 5400 m³/day (6500 m³/day during the busy tourist season).
The overall length of the sewage system was 21 km, and the total retention time was 2.5 hours (approximate time the probiotic products had to impact the water quality).

To control odors in the sewage system and pumping stations in three targeted areas; to reduce the levels of TSS, COD and BOD; and to reduce Total Coliforms and Fecal Coliforms, improving outflow water quality.


SCD Probiotics used were a mixed culture of beneficial microorganisms without genetic manipultion, present in natural ecosystems, physiologically compatiable with each other; when they come in contact with organic matter, they acceleerate the process of decomposition, without allowing putrefacation.
SCD Probiotics in liquid form were applied directly to the manholes of each targeted sector following a strict plan and methodology. In addition, SCD Bokashi Balls (solid microbial concentrate) were dropped in the manholes also according to a strict plan throughout the trial period.

Samples were taken in the three pumping stations every week during 3 months, and lab results were systematically recorded and analyzed to make adjustments on the application plan if necessary.
All samples were taken and analyzed by the personnel of Acuacar/EPA and counter samples were analyzed by a private laboratory. The final results, agreed between the parties, are from the EBAR (pumping station) at a single site (Bocagrande).
Significant results (Table II) were demonstrated and all goals of the project were achieved. Total Suspended Solids (TSS) was reduced 68% Biological Oxygen Demand (BOD) was reduced by 66% Chemical Oxygen Demand (COD) was reducted by 25% Oils & Grease were reduced by 8%. The presence of foul odors in the interior and outside of the manholes showed remarkable reduction
The bacterial load aslo showed significant reductions: Total Coliforms by 91% and Fecal Coliforms by approximately 76% respectively Tables III and IV provide additonal data from the trial period. More information is available by contacting


1This project was managed by Vital Resource Management Pvt. Ltd, Phone: +61 7 4774 6337
2This article is taken from the SCD website, Copyright © 2010 Sustainable Community Development.
3This article is taken from the SCD website, Copyright © 2010 Sustainable Community Development.